The separation of active and inactive forms of heparin

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Abstract

Heparin has been fractionated into two distinct forms. The isolation of these species was accomplished by sucrose density gradient centrifugation of heparin mixed with antithrombin-heparin cofactor. Approximately 13 of this mucopolysaccharide was bound to antithrombin-heparin cofactor and had potent anticoagulant activity. This component was clearly separated from the remaining 23 of the heparin which could not form a stable complex with antithrombin-heparin cofactor and had minimal anticoagulant activity.

References (18)

  • R.F. Highsmith et al.

    J. Biol. Chem

    (1974)
  • J.S. Rosenberg et al.

    J. Biol. Chem

    (1975)
  • R.D. Rosenberg et al.

    J. Biol. Chem

    (1973)
  • M.E. Silva et al.

    J. Biol. Chem

    (1975)
  • T.C. Laurent

    Arch. Biochem. Biophys

    (1961)
  • P. Hovingh et al.

    J. Biol. Chem

    (1970)
  • I. Danishefsky et al.

    J. Biol. Chem

    (1969)
  • T. Bitter et al.

    Anal. Biochem

    (1962)
  • K.M. Brinkhous et al.

    Am. J. Physiol

    (1939)
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