Cancer Letters

Cancer Letters

Volume 9, Issue 4, June 1980, Pages 277-284
Cancer Letters

Research letter
Correlation of inducibility of aryl hydrocarbon hydroxylase with susceptibility to 3-methylcholanthrene-induced lung cancers

https://doi.org/10.1016/0304-3835(80)90018-XGet rights and content

Abstract

C57BL6Cum, DBA2Cum, first filia (F1), and backcross progeny from these 2 parental strains of mice were evaluated for their susceptibility to 3-methylcholanthrene-induced lung cancers. In the crosses among these mice, aryl hydrocarbon hydroxylase (AHH) responsiveness segregated as a single autosomal dominant gene (the Ah locus). AHH responsive mice (Ahb allele) expressed 40–60 units AHH activity/g wet wt liver following intraperitoneal treatment with 3-methylcholanthrene (MCA) compared to AHH non-responsive mice (Ahd allele) which expressed 7–11 units AHH activity/g wet wt liver after MCA treatment. Intratracheal administration of 500 μg MCA for a total of 4 times at weekly intervals yielded a variety of pulmonary cancers, including squamous cell carcinomas, alveolar adenocarcinomas, and adeno-squamous cell carcinomas among mice that survived 1 year after the carcinogen treatment. The AHH responsive C57BL6Cum, F1, and C57BL6Cum × F1 animals were much more susceptible to MCA-induced lung cancers than the AHH non-responsive DBA2Cum × F1 backcross progeny since significantly more lung cancers were found in AHH-responsive progeny than in AHH non-responsive mice. Data support genetic linkage between susceptibility to MCA-induced lung carcinomas and the Ahb allele.

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Present address: Environmental Pathology Services, 809 Viers Mill Road, Rockville, Maryland 20851, U.S.A.

∗∗

Present address: Department of Pathology, School of Medicine, The University of North Carolina at Chapel Hill, Preclinical Educational Building 228H, Chapel Hill, North Carolina 27514, U.S.A.

Present address: Carcinogenesis Testing, Division of Cancer Cause and Prevention, National Cancer Institute, Bethesda, Maryland 20205, U.S.A.

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