Nitroprusside Ameliorates Lung Allograft Reperfusion Injury
Section snippets
Material and Methods
Weight-matched pairs of 10 adult mongrel dogs were used. All animals received humane care in compliance with the “Principles of Laboratory Animal Care” formulated by the National Society for Medical Research and the “Guide for the Care and Use of Laboratory Animals” prepared by the National Academy of Science and published by the National Institutes of Health (NIH publication 85–23, revised 1985).
Results
There were no significant differences among groups with respect to donor weight, recipient weight, flushing times, preservation time, and warm ischemic time (see Table 1). However flushing pressures in group 2 were significantly lower than those in group 1 (group 1, 18.8 ± 1.1 mm Hg; group 2, 15.1 ± 1.1 mm Hg; p < 0.05). Flushing times in group 1 and 2 were 87 ± 7 and 93 ± 8 seconds, respectively. Preservation time of groups 1 and 2 were 21 hours 7 minutes ± 4 minutes and 20 hours 58 minutes ± 7 minutes,
Comment
Despite a large number of studies, the specific pathophysiologic mechanism of ischemia-reperfusion injury remains unclear. However, it has been demonstrated that endothelial dysfunction [4, 7], neutrophil activation [5, 12], oxygen free radicals [13, 14], platelet activation [15], and various cytokines [16] are involved in ischemia-reperfusion injury. The endothelium appears to play a critical role in maintaining vascular homeostasis in ischemic heart [7], lung [8], and intestine [4, 17].
Nitric
Discussion
DR WALTER KLEPETKO (Vienna, Austria): Doctor Yamashita, I congratulate you on this well-designed, timely, and interesting study.
Nitric oxide has played an important role within the last years. Although its mechanism of acting is by far not completely understood, it has a tremendous potential and striking effects on pulmonary circulation and gas exchange as well. This has led to a widespread application of its inhaled form for treatment of patients with different forms of pulmonary
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Therapeutic benefits of nitric oxide in lung transplantation
2023, Biomedicine and PharmacotherapyNebulized nitrite protects rat lung grafts from ischemia reperfusion injury
2013, Journal of Thoracic and Cardiovascular SurgeryCitation Excerpt :Our present data using C-PTIO support the latter findings indirectly, although we did not conduct a direct evaluation using an additional group with the administration of nitrite during reperfusion. In addition to evidence of cytoprotection by inhaled NO in rat lungs,23 much evidence suggests that several NO donors can inhibit lung IRI, including nitroglycerin and nitroprusside.24,25 As opposed to inhaled NO, a benefit of nitrite is that NO generation from nitrite would occur in tissue beds undergoing oxidative stress and at physiologic concentrations, thus minimizing the possibility of deleterious effects.
Suppression of Inflammatory Cytokines During Ex Vivo Lung Perfusion With an Adsorbent Membrane
2010, Annals of Thoracic SurgeryCitation Excerpt :In the current study, corticosteroids were not used because the study aimed to verify basic changes in cytokine levels during prolonged EVLP. Other interventions such as oxygen free radical scavenger [32–34], nitric oxide donor [35–38], and nebulized N-acetyl cysteine [39, 40] have demonstrated efficacy against I/R injury. Addition of these medications may have the potential to improve lung function during prolonged EVLP.
Nitric oxide ventilation of rat lungs from non-heart-beating donors improves posttransplant function
2009, American Journal of TransplantationPost-ischemic Infusion of Atrial Natriuretic Peptide Attenuates Warm Ischemia-Reperfusion Injury in Rat Lung
2009, Journal of Heart and Lung TransplantationInhaled nitric oxide reduces ischemia-reperfusion injury in rat lungs from non-heart-beating donors
2006, Journal of Thoracic and Cardiovascular Surgery
Presented at the Thirty-second Annual Meeting of The Society of Thoracic Surgeons, Orlando, FL, Jan 29–31, 1996.