1887

Abstract

We compared the sequence of an envelope protein gene fragment from 21 temporally distinct West Nile (WN) virus strains, isolated in nine African countries and in France. Alignment of nucleotide sequences defined two groups of viruses which diverged by up to 29%. The first group of subtypes is composed of nine WN strains from France and Africa. The Austral-Asian Kunjin virus was classified as a WN subtype in this first group. The second group includes 12 WN strains from Africa and Madagascar. Four strains harboured a 12 nucleotide in-frame deletion. The loss of the corresponding four amino acids resulted in the loss of the potential glycosylation site present in several WN strains. The distribution of virus subtypes into two lineages did not correlate with host preference or geographical origin. The isolation of closely related subtypes in distant countries is consistent with WN viruses being disseminated by migrating birds.

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1997-09-01
2024-04-20
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References

  1. Adams S. C., Broom A. K., Sammels L. M., Harnett A. C., Howard M. J., Coelen R. J., Mackenzie J. S., Hall R. A. 1995; Glycosylation and antigenic variation among Kunjin virus isolates. Virology 206:49–56
    [Google Scholar]
  2. Anon 1996; Viral meningitidis. Weekly Epidemiological Record 71296
    [Google Scholar]
  3. Besselaar T. G., Blackburn N. K. 1988; Antigenic analysis of West Nile virus strains using monoclonal antibodies. Archives of Virology 99:75–88
    [Google Scholar]
  4. Coia G., Parker M. D., Speight G., Byrne M. E., Westaway E. G. 1988; Nucleotide and complete amino acid sequences of Kunjin virus: definitive gene order and characteristics of the virus-specified proteins. Journal of General Virology 69:1–21
    [Google Scholar]
  5. Deubel V., Nogueira R. M., Drouet M. T., Zeller H., Reynes J. M., Ha D. Q. 1993; Direct sequencing of genomic cDNA fragments amplified by the polymerase chain reaction for molecular epidemiology of dengue-2 viruses. Archives of Virology 129:197–210
    [Google Scholar]
  6. Gaidamovich S. Y., Sokhey J. 1973; Studies on antigenic peculiarities of West Nile virus strains isolated in the USSR by three serological tests. Acta Virologica 17:343
    [Google Scholar]
  7. Hammam H. M., Price W. H. 1966; Further observations on geographic variation in the antigenic character of West Nile and Japanese B encephalitis viruses. American Journal of Epidemiology 83:113
    [Google Scholar]
  8. Hammam H. M., Clark D. H., Price W. H. 1965; Antigenic variations of West Nile virus in relation to geography. American Journal of Epidemiology 82:40–55
    [Google Scholar]
  9. Hayes C. G. 1988; West Nile Fever. In The Arboviruses: Epidemiology and Ecology pp. 59–89 Monath T. P. Edited by Boca Raton, Fla: CRC Press;
    [Google Scholar]
  10. Higgins D. G., Sharp P. M. 1988; CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237–244
    [Google Scholar]
  11. Le Guenno B., Bougermouth A., Dif A., Azzam T., Bouakaz R. 1996; West Nile, a deadly virus?. Lancet 348:1315
    [Google Scholar]
  12. Mathiot C. C., Georges A. J., Deubel V. 1990; Comparative analysis of West Nile virus strains isolated from human and animal hosts using monoclonal antibodies and cDNA restriction digest profiles. Research in Virology 141:533–543
    [Google Scholar]
  13. Monath T. P., Heinz F. X. 1996; Flaviviruses. In Virology pp. 961–1034 Fields B. N., Knipe D. M., Howley P. M. Edited by Philadelphia: Lippincott-Raven;
    [Google Scholar]
  14. Ng M. L., Howe J., Sreenivasan V., Mulders J. J. L. 1994; Flavivirus West Nile (Sarafend) egress at the plasma membrane. Archives of Virology 137:303–313
    [Google Scholar]
  15. Odelola H. A., Fabiyi A. 1976; Antigenic relationships among Nigerian strains of West Nile virus by complement fixation and agar gel precipitation techniques. Transactions of the Royal Society of Tropical Medicine and Hygiene 70:138
    [Google Scholar]
  16. Pierre V., Drouet M. T., Deubel V. 1994; Identification of mosquito-borne flavivirus sequences using universal primers and reverse transcription/polymerase chain reaction. Research in Virology 145:93–104
    [Google Scholar]
  17. Porter K. R., Summers P. L., Dubois D., Puri B., Nelson W., Henchal E., Oprandy J. J., Hayes C. G. 1993; Detection of West Nile virus by the polymerase chain reaction and analysis of nucleotide sequence variation. American Journal of Tropical Medicine and Hygiene 48:440–446
    [Google Scholar]
  18. Price W. H., O’Learly W. 1967; Geographic variation in the antigenic character of West Nile virus. American Journal of Epidemiology 85:84–86
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular and Biological Evolution 4:406–425
    [Google Scholar]
  20. Taylor R. M., Work T. H., Hurlbut H. S., Zizk F. 1956; A study of the ecology of West Nile virus in Egypt. American Journal of Tropical Medicine and Hygiene 5:579–620
    [Google Scholar]
  21. Trent D. W., Kinney R. M., Johnson B. J., Vorndam A. V., Grant J. A., Deubel V., Rice C. M., Hahn C. S. 1987; Partial nucleotide sequence of St. Louis encephalitis virus RNA: structural proteins, NS1, ns2a and ns2b. Virology 256:293–304
    [Google Scholar]
  22. Umrigar M. D., Pavri K. K. M. 1977; Comparative serological studies on Indian strains of West Nile virus isolated from different sources. Indian Journal of Medical Research 65:603
    [Google Scholar]
  23. Wengler G., Castle E., Leidner U., Nowak T., Wengler G. 1985; Sequence analysis of the membrane protein V3of the flavivirus West Nile virus and of its gene. Virology 147:264–274
    [Google Scholar]
  24. Zanotto P. M. D., Gould E. A., Gao G. F., Harvey P. H., Holmes E. C. 1996; Population dynamics of flaviviruses revealed by molecular phylogenies. Proceedings of the National Academy of Sciences, USA 93:548–553
    [Google Scholar]
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