Abstract
Hepatic ischemia-reperfusion injury (I/R) is a serious health problem associated with liver transplantation, resection surgery, and various types of shock especially hemorrhagic shock. In the present investigation, the effect of inhibition of tumor necrosis factor-alpha (TNF-α) using pentoxifylline or infliximab against hepatic I/R injury induced in rats by 45-min ischemia and 1-h reperfusion was studied. It was observed that both pentoxifylline and infliximab-treated groups showed a significantly lower extent and severity of liver injury. This is attributed to (1) a decrease in oxidative stress markers, (2) reduction of the expression of TNF-α, TNF-α type-1 receptors, and nuclear factor kappa B (NF-κB). Thus TNF-α inhibition may be one of the therapeutic interventions to overcome the deleterious effects of I/R on liver via reduction of oxidative stress and inhibition of inflammatory cascade.
MeSH terms
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Alanine Transaminase / blood
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Animals
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Antibodies, Monoclonal / pharmacology
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Antibodies, Monoclonal / therapeutic use*
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Aspartate Aminotransferases / blood
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Gene Expression / drug effects
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Glutathione / metabolism
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Infliximab
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L-Lactate Dehydrogenase / blood
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Liver / drug effects
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Liver / injuries
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Liver / metabolism
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Male
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Malondialdehyde / metabolism
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NF-kappa B / genetics
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NF-kappa B / metabolism
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Oxidative Stress
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Pentoxifylline / pharmacology
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Pentoxifylline / therapeutic use*
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Rats
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Rats, Wistar
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Reperfusion Injury / drug therapy*
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Reperfusion Injury / metabolism
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Tumor Necrosis Factor-alpha / antagonists & inhibitors*
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Antibodies, Monoclonal
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NF-kappa B
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Tumor Necrosis Factor-alpha
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Malondialdehyde
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Infliximab
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L-Lactate Dehydrogenase
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Aspartate Aminotransferases
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Alanine Transaminase
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Glutathione
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Pentoxifylline