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Research ArticleUnder the Microscope

Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue

Stephanie C. Moss, Michael Bates, Patrick E. Parrino and T. Cooper Woods
Ochsner Journal September 2007, 7 (3) 133-136;
Stephanie C. Moss
Laboratory of Molecular Cardiology, Basic Science Research, Ochsner Clinic Foundation, New Orleans, LA
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Michael Bates
Department of Surgery, Ochsner Clinic Foundation, New Orleans, LA
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Patrick E. Parrino
Department of Surgery, Ochsner Clinic Foundation, New Orleans, LA
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T. Cooper Woods
Laboratory of Molecular Cardiology, Basic Science Research, Ochsner Clinic Foundation, New Orleans, LA
Department of Pharmacology & Experimental Therapeutics, Louisiana State University Health Sciences Center, New Orleans, LA
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    Figure 1.

    Representative photomicrograph (63X) demonstrating that the isolated endothelial cells (ECs) stain positive for the EC specific marker CD31. ECs grown on a tissue culture chamber slide were stained with Alexa 647 labeled anti-CD31 antibody (red) and counterstained with the DNA dye DAPI (blue)

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    Figure 2.

    Representative photomicrograph (40X) demonstrating that the isolated vascular smooth muscle cells (VSMCs) exhibit positive staining for α-smooth muscle actin (brown). VSMCs grown on a tissue culture chamber slide were stained for α-smooth muscle actin and counterstained with hematoxylin (blue)

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Sep 2007
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Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue
Stephanie C. Moss, Michael Bates, Patrick E. Parrino, T. Cooper Woods
Ochsner Journal Sep 2007, 7 (3) 133-136;

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Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue
Stephanie C. Moss, Michael Bates, Patrick E. Parrino, T. Cooper Woods
Ochsner Journal Sep 2007, 7 (3) 133-136;
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Keywords

  • Vascular smooth muscle cell
  • endothelial cell
  • mammary artery
  • cell isolation

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