RT Journal Article
SR Electronic
T1 Overexpression of the Progestagen-Associated Endometrial Protein Gene Is Associated With Microphthalmia-Associated Transcription Factor in Human Melanoma
JF Ochsner Journal
JO Ochsner J
FD O. P. Jindal Global University
SP 212
OP 219
VO 11
IS 3
A1 Ren, Suping
A1 Howell, Paul M.
A1 Han, Ying
A1 Wang, Jiexi
A1 Liu, Minxia
A1 Wang, Yan
A1 Quan, Guobo
A1 Du, Wei
A1 Fang, Lei
A1 Riker, Adam I.
YR 2011
UL http://www.ochsnerjournal.org/content/11/3/212.abstract
AB Background We recently reported that the progestagen-associated endometrial protein (PAEP) gene is overexpressed and promotes tumor proliferation and metastasis in human melanoma.Methods To identify the molecules that regulate its expression and oncogenic properties, we analyzed the gene microarray profiling of melanoma samples of serial clinical stage.Results We found that the expression profile of the PAEP gene parallels that of microphthalmia-associated transcription factor (MITF, r  =  0.86), a master regulator of melanocyte development and melanoma progression. This parallelism was further confirmed with semiquantitative reverse transcriptase polymerase chain reaction analysis of melanoma-derived daughter cells. Transfection of melanoma cells with MITF small interfering RNA (siRNA) specifically diminishes PAEP gene expression, whereas PAEP siRNA transfection has no effect on MITF. Furthermore, knockdown of either the MITF or PAEP gene reveals a significant inhibition of tumor cell migration.Conclusions Our data indicate that PAEP expression is regulated in part by MITF and may thus play a role in MITF-mediated cell migration in human melanoma.