Arthroscopy: The Journal of Arthroscopic & Related Surgery
Original ArticleCorticosteroids and Local Anesthetics Decrease Positive Effects of Platelet-Rich Plasma: An In Vitro Study on Human Tendon Cells
Section snippets
Plasma Concentrates
Venous blood was collected from 8 healthy volunteers (2 women and 6 men) with a mean age of 31.7 ± 11.1 years (Institutional Review Board No. 10-204-2). To obtain PRP, 2 separate isolation protocols were used: single spin (PRPSS) and double spin (PRPDS). These protocols were selected to reflect current clinical practice.3
Plasma Concentrates
The preparation of platelets increased the platelet number in the PRPSS group on average to 2.6 times the baseline concentration of whole blood, whereas the platelet number in the PRPDS group was on average 3.3 times the concentration of whole blood. White blood cell concentrations in both the PRPSS group and the PRPDS group were decreased at least 10-fold in comparison with the concentration in whole blood samples (Table 1).
Analysis of Cell Proliferation
There were several significant findings among both the control and
Discussion
This study was designed to provide in vitro data to clinicians who are interested in combining PRP with anesthetics or corticosteroids. With regard to our hypothesis, tenocyte proliferation and viability were increased when PRPSS and PRPDS preparations were added to the tenocyte culture. Conversely, tenocyte proliferation and viability were substantially decreased when anesthetics and corticosteroids were added. Combined treatment, using PRP preparations and anesthetics or corticoids, showed a
Conclusions
The addition of either anesthetics or corticosteroids to PRP resulted in statistically significant decreases in tenocyte proliferation and cell viability. These results suggest that incorporation of anesthetics or corticosteroids, either alone or in combination, with PRP injection may compromise the potentially beneficial in vitro effects of isolated PRP on tendon cells and compromise cell viability at the site of tendon injury.
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Cited by (0)
The authors report the following potential conflict of interest or source of funding in relation to this article: The University of Connecticut Health Center/New England Musculoskeletal Institute has received direct funding and material support for this study from Arthrex Inc., Naples, FL.